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maxsep magnetic separator

UMass Lowell Center for Atmospheric Research/ DigisondeDPS

Parameters MAXSEP, DEVN, ROTATA specify the antenna configuration itself. MAXSEP: This variable specifies the maximum antenna separation of the largest triangle in the seven antenna array configuration (Figure 1a). Namely, MAXSEP is the distance …

Magnetic Separators & Separation Equipment | Bunting Magnetics

Bunting ® Magnetics Co. Magnetic Separation Equipment is used in the food, dairy, grain & milling, chemical, plastics, oil, textile, recycling, and other industries for applications and products similar to yours. With our products, you have a single source of supply for everything you need for efficient separation.

Rapid Immune Recovery and GVHD-like ... - PubMed Central (PMC)

Jul 01, 2009· On the designated infusion date, the T cells were harvested. The beads were removed with a Baxter Fenwal Maxsep® magnetic separator, washed and concentrated with the Baxter Fenwal Harvester System, and resuspended in 100–500 mL 1:1 Plasmalyte A/Dextrose 5%, 0.45% NaCl containing 0.5–1% human serum albumin.

Antenna Array Configuration for DDA software - blogspot.com

MAXSEP: This variable specifies the maximum antenna separation of the largest triangle in the seven antenna array configuration (Figure 1a).Namely, MAXSEP is the distance in meters from antenna 5 to 6, 6 to 7, or 7 to 5. MAXSEP always refers to the outer antennas of the seven-antenna system, even if they are not present (as for the 4-antenna setup of the DPS).

Adoptive transfer of costimulated T cells induces ...

The magnetic beads were removed using a Baxter Fenwal Maxsep magnetic cell separation system. After cell culture, harvest was performed on the day of scheduled cell reinfusion with the Baxter Fenwal cell harvester; the cells were washed and resuspended in Plasmalyte A containing 1% HSA.

The c-Kit Ligand Potentiates the Allogeneic Mixed ...

adhered using a MaxSep magnetic cell separator (Baxter Healthcare Corp, Deerfield, IL) for 10 minutes. The nonadhered cells were collected and subjected to a second magnetic adherence for 10 min- utes. Remaining nonadherent cells were checked for purity by flow cytometry (always showing 53% T cells) and plated at a concentra-

Large-Scale Production of CD4+ T Cells from HIV-1-Infected ...

Mar 27, 2009· Viral load, assessed by DNA PCR for HIV-1 gag, decreased > 10-fold during culture in the absence of antiretroviral agents. Removal of CD3/CD28 beads from the cell suspension was accomplished by passing cells plus beads (3-30 x 109 cells in 2-12 L) over a MaxSep® magnetic separator using gravity-driven flow.

Full text of "USPTO Patents Application 10511818"

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CN1541113A - t - Google …

CN1541113A CNA02812863XA CN02812863A CN1541113A CN 1541113 A CN1541113 A CN 1541113A CN A02812863X A CNA02812863X A CN A02812863XA CN 02812863 A CN02812863 A CN 02812863A CN 1541113 A CN1541113 A CN 1541113A Authority CN China Prior art keywords cells method cell cancer mammal Prior art date 2001-04-27 Application number CNA02812863XA Other …

Efficient clinical-scale enrichment of lymphocytes for use ...

As reported here and by collaborators, monocyte depletion can be achieved by incubating PBMC with Dynabeads, non-porous polystyrene superparamagnetic beads, in the absence of specific antibodies, by capture of adherent cells and removal via a large-scale MaxSep or ClinExVivo ™ magnetic separator. The MaxSep 'semi-closed' method requires ...

A Phase I Trial of CD3/CD28-activated T Cells (Xcellerated ...

moved by processing over a MaxSep Magnetic Separator (Nex-ell Therapeutics, Irvine, CA). Next, the T cells were activated and culture-expandedex vivo in a closed system in an adaptation of a process described previously (10). Briefly, the T cells were costimulated …

Our Facilities | Clinical Cell and Vaccine Production ...

II. Equipment . Critical equipment in the CVPF is monitored continuously by the Rees Centron Environmental Monitoring System that is password protected and can be accessed remotely to provide information on the status of critical equipment, for example, incubators and freezers.

Baxter Fenwel MaxSep Magnetic Cell Separator | Biosurplus

General Description. The Baxter Fenwal MaxSep offers a powerful tool for magnetic cell separation in research settings. This magnetic cell separator removes cell types from dense mixtures found in blood, bone marrow, and other tissue fluids drawn from patients.

Methods for magnetic separation of cells - Henan Mining ...

201911The ability to separate living cells is an essential aspect of cell research Magnetic cell separation methods are among some of the most efficient methods for bulk cell separation With the development of microfluidic platforms within the biotechnology sector, the design of miniaturised magnetic . Live Chat

Isolex Magnetic Cell Selection System Nexell - fda.gov

(Refer to the Operator's Manual supplied with the Isolex Magnetic Cell Selectors for Detailed Instructions For Use) The system components, sample preparation pro c e d u r e and

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Used Baxter Fenwel MaxSep Magnetic Cell Separator (201 ...

Description. The Baxter Fenwal MaxSep offers a powerful tool for magnetic cell separation in research settings. This magnetic cell separator removes cell types from dense mixtures found in blood, bone marrow, and other tissue fluids drawn from patients.

Efficient Clinical Scale Gene Modification via Zinc Finger ...

For clinical scale, anti-CD3/anti-CD28 beads were removed with a MaxSep Magnetic Cell Separator (Baxter), and cells were concentrated and washed with a Baxter Fenwal Harvester (Levine et al., 1998) and subsequently cryopreserved using a controlled rate freezer.

Autologous transplantation of peripheral blood stem cells ...

Request PDF on ResearchGate | Autologous transplantation of peripheral blood stem cells for haematological malignancies. Part II: determination of CD34(+) cells and progenitor colonies | 119 ...

Safety, tumor trafficking and immunogenicity of chimeric ...

Mar 21, 2017· On Day 3, the beads were removed using a Maxsep magnetic bead separator (Baxter, Roundlake, IL, USA) and resuspended in AIM-V medium with IL-2 (200 IU/mL). Transduction with CC49-ζ retroviral supernatant was performed on Days 5 and 7 in AIM-V medium containing IL-2 and polybrene (Aldrich, St Louis, MO, USA) using spinoculation at 800 x g for ...

Complete hematopoietic recovery after the transplant of ...

A negative in vitro purging was performed by an immunomagnetic selection using the MaxSep Magnetic Separation device (Baxter), after the leukapheresis products were incubated first with a cocktail of 3 different monoclonal antibodies against c-erb-B2 receptor and cytokeratin (GP200, GP55, GP40), and than with immunomagnetic beads (Dynabeads ...

CVPF Quality Management Plan - med.upenn.edu

The CVPF Quality Management Plan is modeled in accordance with the standards and practices jointly developed by the International Society for Cellular Therapy (ISCT), the American Association of Blood Banks (AABB), and FACT.

[18F]FHBG PET/CT Imaging of CD34-TK75 Transduced Donor T ...

After removing the Dynabeads using a Fenwell MaxSep Magnetic Separator (Baxter, Deerfield, IL), cells were washed in PBS containing 2 mmol/l ethylenediaminetetraacetic acid and 25% human serum albumin, then centrifuged (510 × g, 7 minutes) to concentrate them.

MAGNETIC SEPARATORS - kanetec.co.jp

Examples of application of magnetic separators in various fi elds Removal of iron and collection of iron powder from various kinds of raw materials and semi-fi nished products are called magnetic separation. Kanetec off ers a wide variety of magnetic separators for use with lump materials, bulk materials, clay-like materials and liquids.

Combined positive and negative cell selection from ...

Using these magnetic bead separation systems, two groups of sequential +/− selection (Isolex 300i version 1/MaxSep and Isolex 300i version 2/MaxSep) and two groups of simultaneous ...

Purging in BCR-ABL-positive acute lymphoblastic leukemia ...

Jan 25, 2000· Dynabeads (Dynal, Hamburg, Germany) and the Baxter MaxSep Magnetic cell separator (Baxter, Deerfield, IL, USA) were used for IMB purging in all patients. From 1992 to …

Enhanced Activation and Expansion of T Cells Using ...

Removal of CD3/CD28 beads from the cell suspension was accomplished by passing cells plus beads (3-30 x 10(9) cells in 2-12 L) over a MaxSep magnetic separator using gravity-driven flow.

SEC Info - Nexell Therapeutics Inc - '10-Q' for 6/30/99 ...

Nexell Therapeutics Inc - '10-Q' for 6/30/99 - EX-10.41 - Quarterly Report - Seq. 3 - Royalty Agreement Between Nexell and Baxter - Accession Number 0001017062-99-001486 - Filing - SEC

Industrial Magnetics Inc. - Magnetic Separators and ...

At Industrial Magnetics, Inc. (IMI), we specialize in industrial strength magnetic separation equipment for the removal of ferrous and non-ferrous metal contaminants from dry or liquid product flows and we are the first manufacturer to offer 52 MgOe rare earth material as a standard in many of our metal separation …